Journal: Journal of Neuroscience Research

Article Title: Lanthionine Ketimine Ethyl Ester Induces Proliferation and Maturation and Regulates Calcium Flux in Primary Mouse Oligodendrocyte Progenitor Cells

doi: 10.1002/jnr.70061

Figure Lengend Snippet: LKE increases OPC maturation. Primary mouse OPCs were incubated with 50 μM LKE for 2 or 4 days, after which the cells were fixed and processed for immunocytochemical staining with the indicated antibodies. Representative images are shown for (A) Olig2; (B) CC1; (C) Olig2 plus CC1; (D) NG2; (E) PLP; and (F) Caspase‐3. The average number of cells per field of view is shown. The % Olig2+/Ki67+ cells is the % of the Olig2+ cells that co‐express CC1. ** p < 0.05; *** p < 0.005 versus same day control cells, 1‐way ANOVA, Tukey's post hoc comparisons. The scale bar shown in panel F is 90 μm and is the same for all panels.

Article Snippet: The primary antibodies used for immunocytochemistry were against caspase‐3 (mouse; 1:400; Cell Signaling, 96,615), the anti‐adenomatous polyposis coli (APC) clone CC1 (mouse; 1:300; EMD Millipore, OP80), the marker of proliferation Ki67 (mouse; 1:200; BD Bio‐sciences, AB_393778), the proteoglycan neuron‐glial antigen 2 (NG2, rabbit; 1:400; END Millipore, AB_5320), the OLG transcription factor Olig2 (rabbit; 1:500; EMD Millipore, AB_9610), and proteolipid protein (PLP, rat; 1:250, AA3‐PLP/DM20).

Techniques: Incubation, Staining, Control

Journal: Cell Reports Medicine

Article Title: Outer radial glia promotes white matter regeneration after neonatal brain injury

doi: 10.1016/j.xcrm.2025.101986

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-CC1 , Millipore , Cat# OP80; RRID: AB_2057371.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Blocking Assay, Staining, Knock-Out, In Situ, Multiplex Assay, Sequencing, Software